extraction and purification of a lectin from red kidney

Extraction and purification of a lectin from small black

Extraction and purification of a lectin from small black kidney bean (Phaseolus vulgaris) using a reversed micellar system Process Biochemistry 48(4) pp 746-752 doi : 10 1016/j procbio 2013 02 007 Access to full text Abstract

Research Article Extraction and Purification of a Lectin

Reversed micelles were used to extract lectin from red kidney beans and factors affecting reverse micellar systems (pH value ionic strength and extraction time) were studied The optimal conditions were extraction at pH 4–6 back extraction at pH 9–11 ion strength at 0 15 M NaCl extraction for 4–6 minutes and back extraction for 8 minutes

Effects of crude red kidney bean lectin

The aim of this study was to obtain information that could help to ease the weaning transition in commercial pig production Before weaning phytohemagglutinin (PHA) in the form of a crude preparation of red kidney bean lectin was fed by gavage to 24 crossbred [(Swedish Landrace x Yorkshire) x Hamps

Purification Biochemical Characterization and Bioactive

The lectins from the common bean Phaseolus vulgaris have been identified and characterized in cultivars from all over the world [12] The most extensively characterized lectins from this species have been purified from the "red kidney" variety The lectin fraction from this bean is composed of

Lectins as Bioactive Proteins in Foods and Feeds

It is known that high doses of uncooked or partially cooked kidney beans can cause food poisoning (Banwell et al 1993 Vasconcelos and Oliveira 2004) The adverse effect of the lectin from red kidney beans (P vulgaris) named PHA is shown by a loss of epithelial resistance leading to acute gastroenteritis Moreover PHA is fatal when ingested at high concentrations

PHYTOHEMAGGLUTININS:ANEWCLASSOFMETALLOPROTEINS

red blood cells (Type A B and 0) were kindly provided by Dr H Oberman University of Michigan All chemicals used were of reagent grade or best quality available 2 1 Purification of the lima bean lectin Finely ground Sieva lima beans Phaseolus lunatus (W Atlee Burpee

Extraction and purification of a lectin from small black

Extraction and purification of a lectin from small black kidney bean (Phaseolus vulgaris) using a reversed micellar system He S -D Shi J Walid E Ma Y et Xue S J (2013) Extraction and purification of a lectin from small black kidney bean (Phaseolus

Extraction and purification of a lectin from small black

Extraction and purification of a lectin from small black kidney bean (Phaseolus vulgaris) using a reversed micellar system He S -D Shi J Walid E Ma Y et Xue S J (2013) Extraction and purification of a lectin from small black kidney bean (Phaseolus

Extraction purification and characterization of lectin

The purpose of the research was to study the purification and characterization of lectin from Phaseolus vulgaris L cv white seeds The lectin was purified by sequence of steps namely first with ammonium sulfate precipitation followed by ion exchange (DEAE cellulose) and gel filtration (sephadex G-200) chromatographies and finally by polyacrylamide electrophoresis (PAGE)

Red kidney bean lectin is a potent cholecystokinin

PURIFICATION OF LECTINS Ground kidney bean (100 g) was extracted by acetic acid and centrifuged the supernatant was dialysed against eight changes of distilled water and freeze dried 17 This preparation contained 35% PHA isolectins mostly of L-type PHA 6–8% trypsin inhibitor and 14–16% amylase inhibitor and uronic acid containing polysaccharides

Extraction and purification of a lectin from small black

Apr 01 2013Highlights Lectin from crude extract of small black kidney bean was extracted The bioprocess was easy to perform using AOT in isooctane reversed micelles The maximum protein recovery (53 28%) and purification factor (8 2-fold) were obtained The efficiency of the process was confirmed by gel electrophoresis analysis The lectin could be pre-purification by one step with a

Extraction purification and characterization of lectin

The purpose of the research was to study the purification and characterization of lectin from Phaseolus vulgaris L cv white seeds The lectin was purified by sequence of steps namely first with ammonium sulfate precipitation followed by ion exchange (DEAE cellulose) and gel filtration (sephadex G-200) chromatographies and finally by polyacrylamide electrophoresis (PAGE)

Isolation and characterization of lectins from kidney

Oct 01 2007The methods used to extract and purify lectins were taken and/or modified from Felsted et al and Ye et al The extraction produce for red kidney bean lectin is shown schematically in Fig 1 One hundred and fifty grams of the dry raw red kidney bean was soaked in 1 L of distilled water and homogenized using a Polytron Mixer (Kinematica USA) for 30 s and then stirred overnight at 4 C

phaseolus vulgaris seed extract 85085

PubMed:Purification biochemical characterization and bioactive properties of a lectin purified from the seeds of white tepary bean (phaseolus acutifolius variety latifolius) PubMed:Extraction and purification of a lectin from red kidney bean and preliminary immune function studies of the lectin and four Chinese herbal polysaccharides

Lectins

Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities Chapters include methods and techniques for serial lectin-affinity chromatography procedure lectin-probed western blot and histochemical analyses quantitative interaction analyses based on equilibrium dialysis isothermal calorimetry surface plasmon resonance

ExtractionandPurificationofaLectinfrom

Reversed micelles were used to extract lectin from red kidney beans and factors affecting reverse micellar systems (pH value ionic strength and extraction time) were studied The optimal conditions were extraction at pH 4–6 back extraction at pH 9–11 ion strength at 0 15M NaCl extraction for 4–6 minutes and back extraction for 8 minutes

Isolation of a Homodimeric Lectin with Antifungal and

A homodimeric lectin adsorbed on Affi-gel blue gel and CM-Sepharose and possessing a molecular weight of 67 kDa was isolated from red kidney beans The hemagglutinating activity of this lectin was inhibited by glycoproteins but not by simple sugars The lectin manifested inhibitory activity on human immunodeficiency virus-1 reverse transcriptase and α-glucosidase

The structure Biology and Application of

Phytohemagglutinin (PHA) the lectin extract from the red kidney bean (Phaseolus Vulgaris) contain potent cell agglutinating and mitogenic activities They play a role in biological recognition phenomena involving cells and proteins towards medical applications The present article is a brief review of the history of lectin in nature

LECTINS AND GLYCOBIOLOGY

our lectins Purification is monitored and final product is assessed by immunoprecipitation with antisera agglutination titre (Red Kidney Bean) seeds 126 4 6 - 8 1 16 Yes Ca ++ Mn Phaseolus vulgaris kidney cells purification of feline T-lymphocytes from peripheral blood Psophocarpus tetragonolobus I

Purification of melibiose

Dec 01 2008Abstract A dimeric 50 kDa melibiose-binding lectin was isolated from the seeds of the cultivar of soybean (Glycine max) called the small glossy black soybean The isolation procedure comprised ion exchange chromatography on Q Sepharose SP Sepharose and Mono Q followed by gel filtration on Superdex 75

Separation and Enrichment of Lectin from Zihua Snap

Sep 22 2017A fast and efficient method based on a polyethylene glycol (PEG) 600/(NH 4) 2 SO 4 aqueous two-phase system for extracting lectin from Zihua snap-bean (Phaseolus vulgaris) seeds was established According to a Box–Behnken design (BBD) involving four factors at three levels each subjected to analysis of variance (ANOVA) and response surface analysis the protein recovery and the purification

Table 3

Extraction and Purification of a Lectin from Red Kidney Bean and Preliminary Immune Function Studies of the Lectin and Four Chinese Herbal Polysaccharides Table 3 Effects of concurrent application of APS and red kidney bean lectin on the spleen and thymus indices and phagocytosis of 𝑀 Φ of mouse

THE EFFECT OF PURIFIED NIGELLA SATIVA LECTIN AGAINST

Extraction of and Purification Lectin: The seeds (50 g) was broken into pieces (approx 1mm) added to a 10-fold volume (150ml) of 50mM phosphate buffer (pH 7 4) and extracted at 4 C for 16h Supernatant obtained by centrifugation (8000rpm for 20min) was then used for lectin purification Proteins in the supernatant were